der Medizinischen Fakultät im Emil-Fischer-Centrum

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Leitung: Prof. Dr. Cord-Michael Becker


C. Villmann and C.-M. Becker (2009)
Cys-loop receptors - Glycine receptors.
In Ion channels -- from Structure to function
J. Kew and C. Davies, eds., 2009, Oxford University Press Canada, in press
C. Villmann and C.-M. Becker (2007)
On the hypes and falls in neuroprotection: Targeting the NMDA receptor
Neuroscientist, 13 (6), 594-615
K. Kashkevich, K. Schiebel (2006)
Polymorphismen in den Prionproteingenen PRNP und PRND und ihre Bedeutung für Prionenkrankheiten.
Nova Acta Leopoldina N.F. , 94, 213-226
K. Kashkevich, K. Schiebel (2005)
Die Genetik von Prionenerkrankungen.
Bioforum , 10, 76-79
Breitinger HG, Becker CM (2002)
The inhibitory glycine receptor-simple views of a complicated channel
Chembiochem, 3, 1042-1052
Becker K, Becker CM, Breitinger HG (2000)
The inhibitory glycine receptor as a model of hereditary channelopathies
Channelopathies; ed. F.Lehmann-Horn; Elsevier (Amsterdam), 199-222
Wassle H, Koulen P, Brandstatter JH, Fletcher EL, Becker CM (1998)
Glycine and GABA receptors in the mammalian retina
Vision Res, 38, 1411-30
Breitinger HG, Becker CM (1998)
The inhibitory glycine receptor, prospects for a therapeutic orphan?
Curr Pharm Des, 4, 315-334

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Shakhman O, Herkert M, Rose C, Humeny A, Becker CM (2003)
Induction by beta-bungarotoxin of apoptosis in cultured hippocampal neurons is mediated by Ca(2+)-dependent formation of reactive oxygen species
J Neurochem, 87, 598-608
Druschky A, Herkert M, Radespiel-Troger M, Druschky K, Hund E, Becker CM, Hilz MJ, Erbguth F, Neundorfer B (2001)
Critical illness polyneuropathy, clinical findings and cell culture assay of neurotoxicity assessed by a prospective study
Intensive Care Med, 27, 686-693
Herkert M, Shakhman O, Schweins E, Becker CM (2001)
Beta-bungarotoxin is a potent inducer of apoptosis in cultured rat neurons by receptor-mediated internalization
Eur J Neurosci, 14, 821-828

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MALDI-TOF-MS and genetic polymorphisms

Meltretter J, Schmidt A, Humeny A, Becker CM, Pischetsrieder M. (2008)
Analysis of the peptide profile of milk and its changes during thermal treatment and storage.
J Agric Food Chem., 56, 2899-906
Meltretter J, Seeber S, Humeny A, Becker CM, Pischetsrieder M. (2007)
Site-specific formation of Maillard, oxidation, and condensation products from whey proteins during reaction with lactose.
J Agric Food Chem., 55,6096-103
Kashkevich, K., Humeny, A., Ziegler, U., Groschup, M.H., Nicken, P., Leeb, T., Fischer, C., Becker, C.M. and Schiebel, K. (2007)
Functional relevance of DNA polymorphisms within the promoter region of the prion protein gene and their association to BSE infection.
Buschmann, A., Gretzschel, A., Biacabe, A.G., Schiebel, K., Corona, C., Hoffmann, C., Eiden, M., Baron, T., Casalone, C. and Groschup, M.H. (2006)
Atypical BSE in Germany--proof of transmissibility and biochemical characterization.
Vet Microbiol, 117, 103-16
Balbus, N., Humeny, A., Kashkevich, K., Henz, I., Fischer, C., Becker, C.M. and Schiebel, K. (2005)
DNA polymorphisms of the prion doppel gene region in four different German cattle breeds and cows tested positive for bovine spongiform encephalopathy.
Mamm Genome, 16, 884-92
Kislinger, T., Humeny, A., Peich, C.C., Becker, C.M. and Pischetsrieder, M. (2005)
Analysis of protein glycation products by MALDI-TOF/MS.
Ann N Y Acad Sci, 1043, 249-59
Reuland, A., Humeny, A., Magener, A., Becker, C.M. and Schiebel, K. (2005)
Detection of loss of heterozygosity by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based analysis of single-nucleotide polymorphisms.
Clin Chem, 51, 636-9
Sander, P., Hamann, H., Drogemuller, C., Kashkevich, K., Schiebel, K. and Leeb, T. (2005)
Bovine prion protein gene (PRNP) promoter polymorphisms modulate PRNP expression and may be responsible for differences in bovine spongiform encephalopathy susceptibility.
J Biol Chem, 280, 37408-14
Kislinger T, Humeny A, Peich CC, Zhang X, Niwa T, Pischetsrieder M, Becker CM (2003)
Relative quantification of N(epsilon)-(Carboxymethyl)lysine, imidazolone A, and the Amadori product in glycated lysozyme by MALDI-TOF mass spectrometry
J Agric Food Chem, 51, 51-57
Humeny A, Beck C, Becker CM, Jeltsch A (2003)
Detection and analysis of enzymatic DNA methylation of oligonucleotide substrates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
Anal Biochem, 313, 160-166
Bonk T, Humeny A, Gebert J, Sutter C, von Knebel Doeberitz M, Becker CM (2003)
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based detection of microsatellite instabilities in coding DNA sequences, a novel approach to identify DNA-mismatch repair-deficient cancer cells
Clin Chem, 49, 552-561
Efferth T, Sauerbrey A, Steinbach D, Gebhart E, Drexler HG, Miyachi H, Chitambar CR, Becker CM, Zintl F, Humeny A (2003)
Analysis of single nucleotide polymorphism C3435T of the multidrug resistance gene MDR1 in acute lymphoblastic leukemia
Int J Oncol, 23, 509-517
Humeny A, Kislinger T, Becker CM, Pischetsrieder M (2002)
Qualitative determination of specific protein glycation products by matrix-assisted laser desorption/ionization mass spectrometry Peptide mapping
J Agric Food Chem, 50, 2153-2160
Bonk T, Humeny A, Sutter C, Gebert J, von Knebel Doeberitz M, Becker CM (2002)
Molecular diagnosis of familial adenomatous polyposis (FAP), genotyping of adenomatous polyposis coli (APC) alleles by MALDI-TOF mass spectrometry
Clin Biochem, 35, 87-92
Humeny A, Schiebel K, Seeber S, Becker CM (2002)
Identification of polymorphisms within the bovine prion protein gene (Prnp) by DNA sequencing and genotyping by MALDI-TOF-MS
Neurogenetics, 4, 59-60
Schmitt C, Humeny A, Becker CM, Brune K, Pahl A (2002)
Polymorphisms of TLR4, rapid genotyping and reduced response to lipopolysaccharide of TLR4 mutant alleles
Clin Chem, 48, 1661-1667
Humeny A, Bonk T, Berkholz A, Wildt L, Becker CM (2001)
Genotyping of thrombotic risk factors by MALDI-TOF mass spectrometry
Clin Biochem, 34, 531-536

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Ion channels

R. Coras, F. A. Siebzehnrubl, E. Pauli, H. B. Huttner, M. Njunting, K. Kobow, C. Villmann, E. Hahnen, W. Neuhuber, D. Weigel, M. Buchfelder, H. Stefan, D. A. Steindler, I. Blümcke. (2010)
Low proliferation and differentiation capacities of adult hippocampal stem cells correlate with memory dysfunction in humans epilepsy.
Brain, 133 (11), 2010:3359-72
C. Villmann, J. Hoffmann, M. Werner, S. Kott, N. Strutz-Seebohm, T. Nilsson, and M. Hollmann. (2008)
Different structural requirements for functional ion pore transplantation suggest different gating mechanisms of NMDA and kainate receptors
J. Neurochem., 107, 453-465
Ulrich, M., Seeber, S., Becker, C.M. and Enz, R. (2007)
Tax1-binding protein 1 is expressed in the retina and interacts with the GABA(C) receptor rho1 subunit.
Biochem J., 401, 429-36
Hoffmann, J., Villmann, C., Werner, M. and Hollmann, M. (2006)
Investigation via ion pore transplantation of the putative relationship between glutamate receptors and K+ channels.
Mol Cell Neurosci, 33, 358-70
Eulenburg, V., Becker, K., Gomeza, J., Schmitt, B., Becker, C.M. and Betz, H. (2006)
Mutations within the human GLYT2 (SLC6A5) gene associated with hyperekplexia.
Biochem Biophys Res Commun, 348, 400-5
Paarmann, I., Frermann, D., Keller, B.U., Villmann, C., Breitinger, H.G. and Hollmann, M. (2005)
Kinetics and subunit composition of NMDA receptors in respiratory-related neurons.
J Neurochem, 93, 812-24
Brill J, Klocke R, Paul D, Boison D, Gouder N, Klugbauer N, Hofmann F, Becker CM, Becker K (2004)
entla, a novel epileptic and ataxic Cacna2d2 mutant of the mouse
J Biol Chem, 279, 7322-7330
Seeber S, Humeny A, Herkert M, Rau T, Eschenhagen T, Becker CM (2004)
Formation of molecular complexes by N-methyl-D-aspartate receptor subunit NR2B and ryanodine receptor 2 in neonatal rat myocard
J Biol Chem, 279, 21062-21068
Brill J, Zeiger U, Welling A, Hofmann F, Becker CM, Becker K (2004)
The a2d-2entla mutation affects processing and function, but not subunit assembly, of voltage gated calcium channels
Seeber S, Becker K, Rau T, Eschenhagen T, Becker CM, Herkert M (2000)
Transient expression of NMDA receptor subunit NR2B in the developing rat heart
J Neurochem, 75, 2472-7
Charton JP, Herkert M, Becker CM, Schroder H (1999)
Cellular and subcellular localization of the 2B-subunit of the NMDA receptor in the adult rat telencephalon
Brain Res, 816, 609-617
Herkert M, Rottger S, Becker CM (1998)
The NMDA receptor subunit NR2B of neonatal rat brain, complex formation and enrichment in axonal growth cones
Eur J Neurosci, 10, 1553-1562

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Glycine receptor

Löhmann Christian, Elena Schachmann, Thomas Dandekar, Carmen Villmann, Cord-Michael Becker (2010)
JNC, E, 0472
Breyer Viola1, Becker Cord-Michael2 and Pischetsrieder Monika1 (2010)
JBC, 133397
Melzer N, Villmann C, Becker K, Harvey K, Harvey RJ, Vogel N, Kluck CJ, Kneussel M, Becker CM. (2009)
Multifunctional basic motif in the glycine receptor intracellular domain induces subunit-specific sorting.
(Epub 2009 Dec 3. PubMed PMID: 19959465; PubMed Central PMCID: PMC2823514.)
J Biol Chem., 285(6):3730-9
Vogel N, Kluck CJ, Melzer N, Schwarzinger S, Breitinger U, Seeber S, Becker CM. (2009)
Mapping of disulfide bonds within the amino-terminal extracellular domain of the inhibitory glycine receptor.
J Biol Chem., 284(52):36128-36.
Vogel, N.; Schiebel, K.; Humeny, A. (2009)
Technologies in the Whole-Genome Age: MALDI-TOF-Based Genotyping
Transfus Med Hemother, 36, 253-262 (DOI:10.1159/000225089)
C. Villmann, J. Oertel, N. Melzer, and Cord-Michael Becker (2009)
Recessive hyperekplexia mutations of the glycine receptor α1 subunit affect cell surface integration and stability
J. Neurochem. , 111(3):837-47
H.-G. Breitinger, C. Villmann, N. Melzer, J. Rennert, U. Breitinger, S. Schwarzinger, C.-M. Becker (2009)
Novel regulatory site within the TM3-4 loop of human recombinant α3 glycine receptors determines channel gating and domain structure
J. Biol. Chem. , 284(42):28624-33
C. Villmann, J. Oertel , Z.-L. Ma-Högemeier, R. Sprengel, M. Hollmann, K. Becker, H.-G. Breitinger and C.-M. Becker (2009)
Functional complementation of Glra1spd-ot, a glycine receptor subunit mutant, by independently expressed C-terminal domains
J. Neurosci., 29(8), 2440-52
Becker K, Breitinger HG, Humeny A, Meinck HM, Dietz B, Aksu F, Becker CM. (2008)
The novel hyperekplexia allele GLRA1(S267N) affects the ethanol site of the glycine receptor.
Eur J Hum Genet., 16, 223-8
Oertel, J., Villmann, C., Kettenmann, H., Kirchhoff, F. and Becker, C.M. (2007)
A novel glycine receptor beta subunit splice variant predicts an unorthodox transmembrane topology. Assembly into heteromeric receptor complexes.
J Biol Chem, 282, 2798-807
Becker, K., Hohoff, C., Schmitt, B., Christen, H.J., Neubauer, B.A., Sandrieser, T. and Becker, C.M. (2006)
Identification of the microdeletion breakpoint in a GLRA1null allele of Turkish hyperekplexia patients.
Hum Mutat, 27, 1061-2
Breitinger, H.G., Lanig, H., Vohwinkel, C., Grewer, C., Breitinger, U., Clark, T. and Becker, C.M. (2004)
Molecular dynamics simulation links conformation of a pore-flanking region to hyperekplexia-related dysfunction of the inhibitory glycine receptor.
Chem Biol, 11, 1339-50
Breitinger U, Breitinger HG, Bauer F, Fahmy K, Glockenhammer D, Becker CM (2004)
Conserved high affinity ligand binding and membrane association in the native and refolded extracellular domain of the human glycine receptor alpha1-subunit
J Biol Chem, 279, 1627-1636
Neumann SB, Seitz R, Gorzella A, Heister A, Doeberitz MK, Becker CM (2004)
Relaxation of glycine receptor and onconeural gene transcription control in NRSF deficient small cell lung cancer cell lines
Brain Res Mol Brain Res, 120, 173-181
Breitinger HG, Villmann C, Rennert J, Ballhausen D, Becker CM (2003)
Hydroxylated residues influence desensitization behaviour of recombinant alpha3 glycine receptor channels
J Neurochem, 83, 30-36
Breitinger HG, Becker CM (2003)
Statistical coassembly of glycine receptor alpha1 wildtype and the hyperekplexia mutant alpha1(P250T) in HEK 293 cells, impaired channel function is not dominant in the recombinant system
Neurosci Lett, 331, 21-24
von Wegerer J, Becker K, Glockenhammer D, Becker CM, Zeilhofer HU, Swandulla D (2003)
Spinal inhibitory synaptic transmission in the glycine receptor mouse mutant spastic
Neurosci Lett, 345, 45-48
Gurrola-Diaz C, Lacroix J, Dihlmann S, Becker CM, von Knebel Doeberitz M (2003)
Reduced expression of the neuron restrictive silencer factor permits transcription of glycine receptor alpha1 subunit in small-cell lung cancer cells
Oncogene, 22, 5636-5645
Humeny A, Bonk T, Becker K, Jafari-Boroujerdi M, Stephani U, Reuter K, Becker CM (2002)
A novel recessive hyperekplexia allele GLRA1 (S231R), genotyping by MALDI-TOF mass spectrometry and functional characterisation as a determinant of cellular glycine receptor trafficking
Eur J Hum Genet, 10, 188-196
Plappert CF, Pilz PK, Becker K, Becker CM, Schnitzler HU (2001)
Increased sensitization of acoustic startle response in spasmodic mice with a mutation of the glycine receptor alpha1-subunit gene
Behav Brain Res, 121, 57-67
Lamp K, Humeny A, Nikolic Z, Imai K, Adamski J, Schiebel K, Becker CM (2001)
The murine GABA(B) receptor 1, cDNA cloning, tissue distribution, structure of the Gabbr1 gene, and mapping to chromosome 17
Cytogenet Cell Genet, 92, 116-121
Breitinger HG, Villmann C, Becker K, Becker CM (2001)
Opposing effects of molecular volume and charge at the hyperekplexia site alpha 1(P250) govern glycine receptor activation and desensitization
J Biol Chem, 276, 29657-29663
Sobetzko D, Sander T, Becker CM (2001)
Genetic variation of the human glycine receptor subunit genes GLRA3 and GLRB and susceptibility to idiopathic generalized epilepsies
Am J Med Genet, 105, 534-538
Busselberg D, Bischoff AM, Becker K, Becker CM, Richter DW (2001)
The respiratory rhythm in mutant oscillator mice
Neurosci Lett, 316, 99-102
Darstein M, Landwehrmeyer GB, Kling C, Becker CM, Feuerstein TJ (2000)
Strychnine-sensitive glycine receptors in rat caudatoputamen are expressed by cholinergic interneurons
Neuroscience, 96, 33-39
Butler MH, Hayashi A, Ohkoshi N, Villmann C, Becker CM, Feng G, De Camilli P, Solimena M (2000)
Autoimmunity to gephyrin in Stiff-Man syndrome
Neuron, 26, 307-312
Saul B, Kuner T, Sobetzko D, Brune W, Hanefeld F, Meinck HM, Becker CM (1999)
Novel GLRA1 missense mutation (P250T) in dominant hyperekplexia defines an intracellular determinant of glycine receptor channel gating
J Neurosci, 19, 869-377
Milani N, Mulhardt C, Weber RG, Lichter P, Kioschis P, Poustka A, Becker CM (1998)
The human glycine receptor beta subunit gene (GLRB), structure, refined chromosomal localization, and population polymorphism
Genomics, 50, 341-345
Nikolic Z, Laube B, Weber RG, Lichter P, Kioschis P, Poustka A, Mulhardt C, Becker CM (1998)
The human glycine receptor subunit alpha3. Glra3 gene structure, chromosomal localization, and functional characterization of alternative transcripts
J Biol Chem, 273, 19708-19714
Kling, C., Koch, M., Saul, B., und Becker, C.-M. (1997)
The frameshift mutation oscillator (Glra1spd-ot) produces a complete loss of glycine receptor a1 polypeptide in mouse central nervous system.
Neuroscience, 78, 411-417
Brune, W., Weber, R.G., Saul, B., von Knebel-D”beritz, M., Grond-Ginsbach, C., Kellermann, K., Meinck, H.-M., und Becker, C.-M. (1996)
A GLRA1 null mutation in recessive hyperekplexia challenges functional role of glycine receptors.
Am. J. Hum. Gen., 58, 989-997
Hartenstein, B., Schenkel, S., Kuhse, J., , Besenbeck, B., Kling, C., Becker, C.-M., Betz, H., und Weiher, H. (1996)
Low level expression of glycine receptor b subunit transgene is sufficient for phenotype correction in spastic mice
EMBO J., 15, 1275-1282
Becker, C.-M. (1995)
Glycine receptors, Molecular heterogeneity and implications for disease.
The Neuroscientist, 1, 130-141
Mlhardt, C., Fischer, M., Gass, P., Simon-Chazottes, D., Gu‚net, J.-L., Kuhse, J., Betz, H., und Becker, C.-M. (1994)
The spastic mouse, Aberrant splicing of glycine receptor b subunit mRNA caused by intronic insertion of L1 element.
Neuron, 13, 1003-1015
Saul, B., Schmieden, V., Mlhardt, C., Gass, P., Kuhse, J., und Becker, C.-M. (1994)
Point mutation of glycine receptor a1 subunit in the spasmodic mouse affects agonist responses.
FEBS Lett., 350, 71-76
Gass, P., Mlhardt, C., Sommer, C., Becker, C.-M., und Kiessling, M. (1993)
NMDA and glycine receptor mRNA expression following transient global ischemia in the gerbil brain.
J. Cerebr. Blood Flow Metab., 13, 337-341
Becker, C.-M., Schmieden, V., Tarroni, P., Strasser, U., und Betz, H. (1992)
Isoform-selective deficit of glycine receptors in the mouse mutant spastic.
Neuron, 8, 283-289
Hoch, W., Betz, H., und Becker, C.-M. (1989)
Primary cultures of mouse spinal cord express the neonatal isoform of the inhibitory glycine receptor
Neuron, 3, 339-348
Becker, C.-M., Hoch, W., und Betz, H. (1988)
Glycine receptor heterogeneity in rat spinal cord during postnatal development.
EMBO J., 7, 3117-3726
Becker, C.-M., Hermans-Borgmeyer, I., Schmitt, B., und Betz, H. (1986)
The glycine receptor deficiency of the mutant mouse spastic. Evidence for normal glycine receptor structure and localization.
J. Neurosci., 6, 1358-1364

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